Composite

Part:BBa_K3635009:Design

Designed by: Emily Blundell   Group: iGEM20_KCL_UK   (2020-10-24)


T7 promoter+RBS+lac operator+Pvfp-5 Beta7xhis-tag+pBluescript II SK+ f1 ori+AmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 564


Design Notes

The T7 promoter has been used to control expression under T7 polymerase. This will allow us to induce transcription with IPTG as our host E.coli will contain the lac cassette in its genome.We have further added the lac operator sequence downstream of the RBS to eliminate any background transcription. A His Tag has been added to give 7 histidines at the C terminus of the protein to allow for purification.


Source

The genomic sequence for this part has been derived from part BBa_k3635008 and part BBa_K3635000

References